Effects of low - dose capsaicin on l - type calcium current in guinea pig ventricular myocytes 小劑量辣椒素對豚鼠心室肌細胞l -型鈣電流的影響
Effect of urocortin on l - type calcium currents in adult rat ventricular myocytes pharmacol 觀察urocortin2對于細胞內ca2濃度,再利用全細胞膜片鉗
The purposes of the present study were to investigate ( 1 ) the hemodynamic effects of agmatine in anaesthetized dahl salt - sensitive ( ds ) hypertensive and dahl salt - resistant ( dr ) rats ; ( 2 ) the effect of agmatine on vascular tension in the isolated aortic artery of rats and the underlying receptor mechanism ; ( 3 ) the effects of local injection of agmatine on femoral , renal , and mesenteric vascular beds by constant flow perfusion method ; ( 4 ) the effect of agmatine on l - type calcium current ( / ca - t ) in rat ventricular myocytes with whole - cell configuration of the patch - clamp technique ; ( 5 ) the effects of agmatine on free intracellular calcium concentration ( ca2 + d of isolated rat ventricular myocytes ( 3 )采用后肢、腎臟和腸系膜動脈在體恒流灌注法,觀察向灌流環路中直接注射胍丁胺的血管效應。 ( 4 )應用全細胞膜片箝技術,觀察胍丁胺對大鼠心室肌細胞l -型鈣通道電流( i _ ( ca - l ) )的影響。 ( 5 )用fluo3 - am負載分離的大鼠心室肌細胞后,由激光共聚焦法測定單個心室肌細胞[ ca ~ ( 2 + ) ] _ i的熒光強度,觀察胍丁胺對分離大鼠心室肌細胞內游離鈣濃度( [ ca ~ ( 2 + ) ] _ i )的影響。